Measurement of serum triglycerides by thin-layer chromatography and infrared spectrophotometry
نویسندگان
چکیده
منابع مشابه
Simultaneous Determination of Triglycerides and Cholesterol Esters in Serum by Infrared Spectrophotometry.
With the use of a high-resolution grating infrared spectrophotometer, it is possible to analyze mixtures of cholesterol esters and triglycerides on the basis of the difference in their ester carbonyl absorption frequencies. In the analysis of serum lipids, the preliminary removal of phospholipids is accomplished by a simple batch adsorption on silicic acid. Infrared absorbance measurements are ...
متن کاملSimultaneous determination of triglycerides and cholesterol esters in serum by infrared spectrophotometry
With the use of a high-resolution grating infrared spectrophotometer, it is possible to analyze mixtures of cholesterol esters and triglycerides on the basis of the difference in their ester carbonyl absorption frequencies. In the analysis of serum lipids, the preliminary removal of phospholipids is accomplished by a simple batch adsorption on silicic acid. Infrared absorbance measurements are ...
متن کاملQuantitative Determination of Plasma Free Fatty Acids and Triglycerides by Thin-layer Chromatography.
QUANTITATIVE ANALYSIS of lipids by elution from thinlayer chromatograms (1) usually requires milligram amounts. Densitometry on thin-layer plates has been successfully used for quantitative analysis by Zollner et al. (2) for plasma cholesterol esters and by Blank et al. (3) for mixtures of mono-, diand triglycerides, neutral and phospholipids. The relation of spot area to amount of materials af...
متن کاملDetermination of carotenes by thin-layer chromatography.
The carotenoids of yellow, red and tangerine tomato fruits were separated and identified by thin-layer chromatography (TLC). β-carotene is extracted with a mixture content hexan and acetone. The various systems of adsorbents and solvents for TLC of carote and tomato carotenes were examined, and it was revealed that the most suitable TLC system for separation and identification was silica gel pl...
متن کاملIdentification of Barbiturates by Thin-layer Chromatography.
in cases of overdose and identification of the actual barbiturate concerned may be impossible because of the closely similar Rf values of this group. Jackson (1960) described treatment with hot sulphuric acid before paper chromatography to distinguish between barbiturates of similar chromatographic mobility. The inert nature of the silica gel supporting medium used in thin-layer separations ena...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Lipid Research
سال: 1963
ISSN: 0022-2275
DOI: 10.1016/s0022-2275(20)40283-4